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Porphyromonas gingivalis Search Results

Record: 1 of 1  
MiniMap tRNA-Lys-2 IGR1534 IGR1542 IGR1539 IGR1543 IGR1536 IGR1535 IGR1541 IGR1544 IGR1540 IGR1537 IGR1538 ISPg2, - PG1795 ISPg5, - PG1797 PG1793 ISPg5, - PG1796 pyrD, - PG1794 dfrA,folA, - PG1800 bioD, - PG1792 ISPg1, - PG1798 thyA, - PG1799 dapA, - PG1791 hisS, - PG1801 ISPg2, - PG1795 ISPg5, - PG1797 PG1793 ISPg5, - PG1796 pyrD, - PG1794 dfrA,folA, - PG1800 bioD, - PG1792 ISPg1, - PG1798 thyA, - PG1799 dapA, - PG1791 hisS, - PG1801 Type: IS element, Name: ISPg1 fragment - 3 Type: IS element, Name: ISPg5 - 8 Type: IS element, Name: ISPg5 - 8 ISPg2, - PG1795 ISPg5, - PG1797 PG1793 ISPg5, - PG1796 pyrD, - PG1794 dfrA,folA, - PG1800 ISPg1, - PG1798 thyA, - PG1799 dapA, - PG1791 hisS, - PG1801 bioD, - PG1792


LANL Gene ID: PG1796

GenBank Locus Tag: PG2057

DNA Molecule Name:
1  

GenBank ID:
34541641

Gene Name:
ISPg5  

Definition:
ISPg5 transposase

Cellular Location:
Cytoplasm [Evidence]

Gene Start:
2156040

Gene Stop:
2155105

Gene Length:
936

Molecular Weight*:
36304

pI*:
10.30

Net Charge*:
17.47

EC:
 

Functional Class:
Transposon functions  

Gene Ontology:
Biological process
  GO:0015074    DNA integration

Molecular function
  GO:0003677    DNA binding


Pathway: pathway table

Primary Evidence:
Califano,J.V., Kitten,T., Lewis,J.P., Macrina,F.L., Fleischmann,R.D., Fraser,C.M.,Duncan,M.J. and Dewhirst,F.E. Characterization of Porphyromonas gingivalis insertion
sequence-like element ISPg5.
Infect. Immun. 68 (9), 5247-5253 (2000)

Comment:
One of eleven ISPg5 elements in the genome (see Paralogs). Califano et al. report considerable strain diversity of these (Infect. Immun. 68 (9), 5247-5253 (2000)).

Member of the IS3 family of insertion elements. See PG1797 for associated ORF.

TIGR ID: PG2057

Human Oral Microbiome Database:
View in HOMD Genome Viewer

Blast Summary:  PSI-Blast Search
PG1796 is equivalent to the previously reported gb|AAF69128.1| in GenBANK.

Numerous significant hits in gapped BLAST to transposase proteins; e.g. residues 91-213 are 56% similar to transposase homolog (U67062) of Bacteriodes thetaiotaomicrom, residues 64-287 are 32% similar to InsB (AF153317) of Shigella dysenteriae, residues 64-268 are 33% similar to Hp1 (AF081284) of E.coli.

Top Blast Hits:  Updated monthly
Click here to view the entire PsiBlast results.
 gi|34541641|ref|NP_906120.1|  ISPg5, transposase Orf2 [Porph...   624   e-177
 gi|34540696|ref|NP_905175.1|  ISPg5, transposase Orf2 [Porph...   619   e-176
 gi|34540399|ref|NP_904878.1|  ISPg5, transposase Orf2 [Porph...   619   e-176
 gi|118200270|dbj|BAF37077.1|  transposase Orf2 [Porphyromona...   618   e-175
 gi|34539915|ref|NP_904394.1|  ISPg5 transposase Orf2 [Porphy...   617   e-175
 gi|34540277|ref|NP_904756.1|  ISPg5, transposase Orf2 [Porph...   614   e-174
 gi|34541287|ref|NP_905766.1|  ISPg5, transposase Orf2 [Porph...   613   e-174
 gi|34539888|ref|NP_904367.1|  ISPg5 transposase Orf2 [Porphy...   612   e-173
 gi|34541341|ref|NP_905820.1|  ISPg5, transposase Orf2 [Porph...   610   e-173
 gi|34540248|ref|NP_904727.1|  ISPg5, transposase Orf2 [Porph...   609   e-173


InterPro Summary:  InterProScan

InterPro
IPR001584
Domain
Integrase, catalytic core
PF00665 [110-273]T 3.4e-30 PF00665 rve rve
PS50994 [110-276]T 18.197 PS50994 INTEGRASE INTEGRASE
noIPR
unintegrated
unintegrated
G3DSA:3.30.420.10 [113-231]T 8.9e-06 G3DSA:3.30.420.10 no description no description


COGS Summary:  COGS Search
No hit to the COGs database.
Blocks Summary:  Blocks Search
***** PF01836 (Transposase) with a combined E-value of 6.4e-22.
    PF01836B    103-154
    PF01836C    181-190
    PF01836D    215-222
    PF01836E    253-272


 ProDom Summary:  Protein Domain Search
No significant hit to the ProDom database.

Paralogs:  Local Blast Search
PG1796 is essentially identical to PG0533, PG1247, PG0841, PG0034, and PG1436, and is paralogous to PG0418, PG1861, PG0007, PG1491, and PG0391, all ISPg5 transposases.

Pfam Summary:  Pfam Search
Residues 110 to 273 (E-value = 3.2e-30) place PG1796 in the rve family which is described as Integrase core domain (PF00665)

 Structural Feature(s):
Feature Type  Start  Stop
No predicted structural features.  
  

Top PDB Hits:
-55% similar to PDB:1DZB CRYSTAL STRUCTURE OF PHAGE LIBRARY-DERIVED SINGLE-CHAIN FV FRAGMENT 1F9 IN COMPLEX WITH TURKEY EGG-WHITE LYSOZYME (E_value = );

Gene Protein Sequence:
LLGFSRQAFYKRHLNDLARHEEDVLCSSIIQYCWHLRQAEHLPQAGFREL
MVLCQQYFGPKFTLGRDRFCALLRRHGMMLRKRSVRPRTTNSRHRLYKYE
DLLNTEPKFVPQRPGELLVADITYVAYQDGFAYLSLLTDAYSRCIVGYCL
HPTLEVEGCLNALHQAFAFYDQHQIDTSNMIHHSDRGIQYAGKSYTDLLH
GRGCRISMTQTGDPLHNALAERMNNTLKNSWHISSSKQSFDQALLSVDRA
VRMYNEARPHQALRAKTPMQVIAPESKNPLLTRREHGPEIAPELYRRMNV
RQRANFARVNRN

Gene Nucleotide Sequence:  Sequence Viewer
CTGCTTGGCTTTAGCCGTCAGGCCTTTTATAAACGTCATCTCAACGATTT
AGCTAGGCACGAGGAGGATGTCCTATGCAGTAGTATCATCCAATATTGTT
GGCACTTAAGACAAGCAGAGCACCTACCTCAAGCCGGCTTCCGAGAGCTC
ATGGTGCTTTGTCAGCAATACTTCGGCCCCAAGTTCACGCTTGGGCGCGA
TCGCTTTTGCGCCCTACTCAGACGCCATGGTATGATGCTGCGCAAACGGT
CTGTGCGCCCTCGGACAACCAACTCTCGGCATAGGCTCTACAAGTATGAA
GACCTGCTCAATACAGAGCCTAAGTTTGTGCCTCAAAGACCTGGGGAACT
CCTTGTAGCAGACATCACCTACGTGGCTTACCAAGATGGGTTTGCCTATC
TCTCCCTACTGACCGATGCCTATAGTCGCTGTATCGTAGGGTATTGCTTA
CATCCTACCCTAGAGGTTGAAGGCTGTTTGAATGCTCTGCATCAAGCCTT
TGCTTTCTACGATCAGCATCAAATTGATACGAGCAATATGATTCATCATA
GCGACCGAGGCATTCAGTATGCCGGTAAGAGCTACACCGATCTGTTGCAT
GGGCGAGGCTGTCGCATCAGCATGACTCAGACGGGAGATCCTCTGCATAA
TGCATTGGCTGAGCGGATGAACAATACGCTCAAGAACTCGTGGCACATCT
CCTCTTCGAAGCAGTCTTTTGATCAAGCACTCCTTTCTGTAGACCGAGCC
GTGCGCATGTACAACGAGGCGCGTCCACATCAAGCTCTAAGGGCGAAAAC
GCCTATGCAAGTCATTGCGCCGGAGTCTAAAAATCCGTTACTAACGAGGA
GGGAGCATGGGCCAGAGATTGCCCCCGAGCTATACCGAAGGATGAATGTC
AGACAAAGAGCTAACTTTGCTCGTGTCAACCGAAAT

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