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Porphyromonas gingivalis Search Results

Record: 1 of 1  
MiniMap IGR1628 IGR1623 IGR1625 IGR1626 IGR1629 IGR1624 IGR1627 PG1894.1 PG1891 PG1894 surE, - PG1890 lpxB, - PG1889 gluP, - PG1897 PG1893 glyS, - PG1892 PG1895.1 ISPg1, - PG1895 PG1894.1 PG1891 PG1894 surE, - PG1890 lpxB, - PG1889 gluP, - PG1897 PG1893 glyS, - PG1892 PG1895.1 ISPg1, - PG1895 Type: IS element, Name: ISPg1 fragment - 3 Type: IS element, Name: ISPg1 fragment - 3 PG1891 PG1894 surE, - PG1890 lpxB, - PG1889 gluP, - PG1897 PG1893 glyS, - PG1892 PG1894.1 PG1895.1 ISPg1, - PG1895


LANL Gene ID: PG1893

GenBank Locus Tag: PG2167

DNA Molecule Name:
1  

GenBank ID:
34541739

Gene Name:


Definition:
possible outer membrane-associated protein P51

Cellular Location:
Cytoplasm, Periplasm [Evidence]

Gene Start:
2273636

Gene Stop:
2272200

Gene Length:
1437

Molecular Weight*:
53513

pI*:
9.70

Net Charge*:
8.40

EC:
 

Functional Class:
Cell envelope  

Gene Ontology:
Cellular component
  GO:0009279    cell outer membrane


Pathway: pathway table

Primary Evidence:
See Fimbrillins

Veith,P., Talbo,G., Slakeski,N., Dashper,S., Moore,C.,Paolini,R., Reynolds,E., Major outer membrane proteins and proteolytic processing of RgpA, and Kgp of Porphyromonas gingivalis W50, Biochem J. 2002 (in press).

Ross BC, Czajkowski L, Hocking D, Margetts M, Webb E, Rothel L, PattersonM, Agius C, Camuglia S, Reynolds E, Littlejohn T, Gaeta B, Ng A, Kuczek ES, Mattick JS, Gearing D, Barr IG, Identification of vaccine candidate antigens from a genomic analysis of Porphyromonas gingivalis, Vaccine 2001 Jul 20;19(30): 4135-42, PMID: 11457538.

Ross,B., Barr,I., Patterson,M., Agius,C., Rothel,L., Margetts,M., Hocking,D. and Webb,E. Porphyromonas gingivalis polypeptides and nucleic acids. Patent: Australia (AU 98/01023)-PCT 10-DEC-1998; CSL Ltd; 45 Poplar Road; R&D; Parkville, Victoria; Australia.

Arai M, Hamada N, Umemoto T.
Purification and characterization of a novel secondary fimbrial protein from Porphyromonas gingivalis strain 381.
FEMS Microbiol Lett. 2000 Dec 1;193(1):75-81.
PMID: 11094282

Comment:
Ross, et al.(2001) select PG1893 as one of fifteen vaccine candidate antigens, based on in vitro and in vivo laboratory studies of 120 candidates screened by bioinformatics techniques from the whole W50 genome.

Corresponds to the "secondary fimbrial protein" reported by Arai et. al. (2000). See also PG1864, PG0916, PG0627. Reported in Genbank AAD33939 gene product is immunoreactive 53 kD antigen PG123, which is reactive with sera from human periodontitis patients and with sera from protected rats.

Veith et al. (2002) identified this protein by two-dimensional gel electrophoresis and peptide mass fingerprinting of outer membranes prepared from strain W50. The peptide mass data indicate that the mature N-terminus of this protein is pyroglutamate.

TIGR ID: PG2167

Proteomic Data: Proteomic Data Search
TIGR Annotationimmunoreactive 53 kDa antigen PG123, GroupT (unknown function)
ORF #PG-PP/PG-PPC1PG-PP/PG-PPC2
Spectral CountPeptide IntensitySpectral CountPeptide Intensity
ProqValProqValProqValProqVal
TIGRPG21670.842.5e-110.250.091.1400.250.09
LANLPG18931.791.192.211.77
ConsensusSP1IP1SP2IP2

Human Oral Microbiome Database:
View in HOMD Genome Viewer

 Blast Summary:  PSI-Blast Search
PG1893 is identical to a previously sequenced P.gingivalis protein in Genbank, AAD33939. Residues 28-475 of PG1893 are 32% similar to a previously sequenced P.gingivalis protein in Genbank, BAA33066.



 Top Blast Hits:  Updated monthly
Click here to view the entire PsiBlast results.
 gi|34541739|ref|NP_906218.1|  immunoreactive 53 kDa antigen ...   917   0.0  
 gi|150009193|ref|YP_001303936.1|  hypothetical protein BDI_2...   259   3e-67
 gi|60681662|ref|YP_211806.1|  hypothetical protein BF2183 [B...   239   2e-61
 gi|53713414|ref|YP_099406.1|  hypothetical protein BF2125 [B...   238   1e-60
 gi|34541708|ref|NP_906187.1|  60 kDa protein [Porphyromonas ...   236   2e-60
 gi|48857023|gb|AAT47117.1|  outer membrane protein [Porphyro...   236   3e-60
 gi|118200272|dbj|BAF37079.1|  hypothetical protein [Porphyro...   235   4e-60
 gi|3599591|dbj|BAA33066.1|  60kDa protein [Porphyromonas gin...   235   5e-60
 gi|118200282|dbj|BAF37088.1|  hypothetical protein [Porphyro...   234   8e-60
 gi|156860376|gb|EDO53807.1|  hypothetical protein BACUNI_024...   234   8e-60


InterPro Summary:  InterProScan

InterPro
IPR006665
Domain
OmpA/MotB
PD000930 [195-260]T 0.0 PD000930 OmpA/MotB OmpA/MotB
PS51123 [184-311]T 0.0 PS51123 OMPA_2 OMPA_2
noIPR
unintegrated
unintegrated
G3DSA:3.30.1330.60 [179-285]T 2.80000043118237E-4 G3DSA:3.30.1330.60 G3DSA:3.30.1330.60 G3DSA:3.30.1330.60
SSF103088 [190-307]T 1.9E-9 SSF103088 SSF103088 SSF103088
SSF81901 [403-472]T 7.6E-6 SSF81901 SSF81901 SSF81901


COGS Summary:  COGS Search
BeTs to 4 clades of COG1360
COG name: Flagellar motor protein MotB
Functional Class: N
The phylogenetic pattern of COG1360 is -----Qv-EBrHUJ--OLinx
Number of proteins in this genome belonging to this COG is 5
Blocks Summary:  Blocks Search
***** IPB001145 (Bacterial outer membrane protein) with a combined E-value of 2.6e-06.
    IPB001145B    194-220
    IPB001145C    236-271


 ProDom Summary:  Protein Domain Search
Residues 28-475 are 32% similar to a (FIMBRILIN) protein domain (PD142733 which is seen in O07074_PORGI.


Paralogs:  Local Blast Search
Residues 28-475 are 32% similar to PG1864, a 60 kDa protein. Weaker similarities are seen to PG0916, a hypothetical protein (possible immunoreactive antigen).

Pfam Summary:  Pfam Search
No significant hits to the Pfam 11.0 database

 Structural Feature(s):
Feature Type  Start  Stop
gram negative signal  
1  
20
cleavable signal  
1  
22
non-globular  
289  
337

Top PDB Hits:
No significant hits to the PDB database (E-value < E-10).

Gene Protein Sequence:
MKRIQLTLIALFAAVAGLVAQNAYEGVISYKISLDKTGNKVVLNGAADMS
NLKLKSTQMIIVTPILRSEDGTSRVEFPSVVITGRNRTKALKREIAFSSA
LPQAKHAAQYIRRHNGKSEQFAFTGEHAYASWMMDAKFVVREEVRGCAKC
PVGLSSNIVPFDPLFNPAEAPYLLAHITPAEEVEKQRESSFDAYINFKVN
KADVLPEYRNNKAELEKIKEFVSTVKANPNYSVNKMIIEGFASPEASIAH
NKALSERRAKRLAEELVRKYGKTLPNITTEFGGEDWKGLKLAIEKSDIAD
RDRVLEIINSDKYADDDAREQALKQLSSYRYILDQIYPNLRRNTITMGYI
VRDYTLEEAREIIKTAPKELSEAEMYRVAMSYPEGHQERLFALNTTLKYF
PESVTGRINLAVAAFNGGDVQQAIALLSPIQTEKGVSNILGAAYARTGDF
ARAETFFRKAVAEGDANAQRNLDMLLGKK

Gene Nucleotide Sequence:  Sequence Viewer
ATGAAAAGAATACAACTAACTCTTATCGCTCTCTTCGCCGCTGTTGCCGG
TTTGGTCGCTCAAAATGCTTACGAGGGAGTAATTTCATATAAAATTTCGT
TGGACAAAACCGGAAACAAGGTTGTACTGAATGGTGCGGCAGATATGAGT
AATTTAAAGCTCAAGAGCACTCAGATGATCATTGTTACGCCTATTCTTCG
TTCAGAAGATGGTACCAGCCGGGTGGAATTTCCTTCGGTAGTCATTACAG
GCCGCAATAGAACAAAAGCTCTCAAGCGTGAAATCGCATTTAGTTCGGCT
TTGCCCCAAGCAAAACATGCAGCTCAATACATTCGCCGTCATAATGGGAA
GAGCGAGCAGTTTGCTTTTACAGGAGAACATGCTTATGCATCATGGATGA
TGGATGCCAAGTTTGTGGTTCGTGAGGAGGTACGAGGTTGTGCTAAATGC
CCTGTAGGTCTCTCGAGTAATATTGTTCCTTTTGATCCACTCTTCAATCC
GGCAGAGGCTCCTTATTTGTTGGCACACATTACTCCGGCAGAAGAAGTGG
AAAAACAGCGAGAGTCCAGCTTCGATGCTTATATCAACTTCAAAGTCAAT
AAGGCAGATGTCCTTCCTGAGTATCGCAACAATAAGGCGGAGTTAGAGAA
AATCAAAGAATTTGTAAGCACCGTTAAGGCTAATCCAAACTATTCGGTCA
ATAAAATGATCATCGAAGGGTTTGCTTCTCCCGAGGCTTCAATAGCCCAC
AATAAGGCTTTGTCGGAGCGCCGTGCTAAAAGACTCGCGGAAGAATTGGT
GCGTAAGTATGGCAAAACATTGCCGAATATAACCACTGAATTCGGCGGTG
AAGATTGGAAGGGGCTGAAACTGGCTATCGAAAAGAGTGATATAGCCGAT
CGTGACCGCGTATTGGAGATAATCAACTCCGATAAATATGCCGATGATGA
TGCACGTGAACAGGCTCTGAAGCAACTTTCGTCTTATCGTTATATCTTGG
ATCAGATCTATCCGAATTTGCGTCGCAATACGATAACCATGGGGTATATC
GTTCGTGATTATACCCTCGAAGAAGCTCGTGAAATCATTAAGACTGCTCC
GAAAGAACTTAGTGAGGCCGAAATGTACCGTGTGGCAATGTCTTATCCTG
AGGGGCACCAAGAGCGTTTGTTTGCTCTGAATACGACCCTTAAGTATTTC
CCTGAAAGTGTAACGGGCCGAATCAATTTGGCTGTAGCCGCTTTTAATGG
TGGAGACGTTCAACAGGCAATTGCTCTGTTGAGTCCGATTCAGACAGAAA
AGGGTGTAAGCAATATCCTTGGAGCTGCTTATGCTCGTACGGGAGATTTT
GCTCGTGCCGAAACCTTCTTCCGTAAGGCCGTTGCAGAAGGAGATGCAAA
TGCGCAGCGCAACCTCGATATGCTGCTTGGCAAAAAG

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