10 20 30 40

....|....|....|....|....|....|....|....|....

PG0254 INISVNGGEFLSVMGPSGCGKSTLLN-VMGLLDR-PTTGTVEIL

PG0618 VSLKVDKGEFIAIMGPSGCGKSTLLN-ILGLLDN-PTSGIYKLD

PG1477 VDIAIDRGEIVSIVGTSGAGKTTLLQ-ILGTLDR-ADSGELRID

PG0921 ISATFETGKTNLIIGRSGAGKTVLLKCIIGLIA--PTSGEVLYD

PG1917 LNLTLSAGDFVYLIGSVGSGKSTLLKALYAEVP--ISAGYARVI

PG1211 LSLNIPKGEIFAITGPSGCGKSTLLHVLAGVLT--EYEGEVLFD

PG1533 YSLDIVRGEFLGIVGRNGGGKSTLVKAIIGLLP--LRSGSLTFY

PG0605 LEAHLHAGQLTALLGVNGAGKSTLMRTMAGFMK--PLSGQIHLL

PG0583 IDLSMLNGELVMLMGPNGCGKSTLMHTIAGLLP--PISGNITIA

PG0566 VSIEVRQGEIVGLLGPNGAGKTTTFYMTTGLVV--PNEGRIFLN

PG1049 IDFSIPSGQTYALVGTSGGGKTTIARLIPRFWD--ATQGHIRIG

PG1048 VSFTAKQGEVTALVGPSGGGKSTSAKLAARFWD--IDGGKIFLG

PG1497 VCLTIPKGKTVALVGQSGSGKSTIVDLLPRFYD--LQQGRVLLD

PG0903 LNLDLPKGSITGLLGRNGEGKTTLLKLLCGQLL--RKDGNLTVL

PG0844 VSISVRKGRVFGLLGPNGAGKTSLIRIINRITA--PDEGEVIFD

PG1923-N VSFVINRRDRIALVGKNGAGKSTLLKLIAGMEE--PTSGHIARP

PG1929-N VNLTFTPGNCYGIIGANGAGKSTMLRVISKQLD--PTRGTVTLG

PG1084-N LSFSIERGEKVGLIARNGNGKTTLMNILTGIDS--PDSGEVIYQ

PG1923-C ATYTIERGEKVAFVGKNGAGKSTMVKCIMGELT--DYTGKLELG

PG1084-C FDYIFSRYDKVGIVGPNGVGKTTFLRLLLGEEK--PDSGYFDVG

PG0233 INLEINAGEIHAIMGPNGSGKSTLSSVLVGHPSFEVTEGEVTFN

Walker A

10 20 30 40 50 60 70 80

....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|

PG0254 YPQQLSGGQCQRVAIARAIVGNPEIILADEPTGNLDSKMGAEVMSILHRLNTEDGRTIVMVTHNELQARET--SRTIRMFDGHQV

PG0618 FPNQLSGGQQQRVAIARAVVANPKLILADEPTGNLDSKNGADVMELLRGLNRE-GATIVMVTHSEHDARSA--GRIINLFDGKIR

PG1477 KPSELSGGEKQRIAVARALVNHPAIILADEPSGSLDSAHKEELHALFFRLCREMGQTFLIVTHDEKLAAGT--DRILHMRDGLLF

PG0921 YPSEISGGMMKRAAIARAIALGPKYLFCDEPNSGLDPKTSLVIDDLIHSLTQEHGITTVVNTHDMNSVKTIG-DKIIYIRHGSKE

PG1917 RPHELSGGEQQRVGIARALLAKPALILADEPTGNLDSVTGLQIASLLYEISKQ-GTAVLMSTHNSSLLSHLP-ARTLAVRKNGDA

PG1211 YPKDLSGGQRQRVALARVFLQRPQLLLLDEAFAALDMLTAEKSRQLFLGLWDKHRVTTVMVTHNVEEAVAMS-SRVAVIGGTPGR

PG1533 PISDLSGGQLQRVLLARAIVSCPELLVLDEPNSYLDESAEALVQQTVSQRHKD-GATILLISHDRESIGQQA-DRILSLD-----

PG0605 ELTTLSDGERQKVVIAKALAQQTPIIFLDEPTAFLDYPSKMELLHLLHSLAHDTGRTIFLSTHDLDAILPMA-DRVWLLDKERSF

PG0583 QYGELSDGEKQRVMIARALAQQTPVMLLDEPTAHLDLPSRLEVIIMLRELARKTNKSILVSTHEMDLALQWA-DTVWLMNSQGEI

PG0566 LGDRLSGGERRRAEIARCLAISPRFIMLDEPFAGVDPIAVQDIQAIVAKLKDK-NIGILITDHNVHETLSIT-DRAYLLFEGKVL

PG1049 DGTYLSGGEQQRIALARAILKDAPIVVLDEATAFADPENEKLIQQALHELTKG--KTVLMIAHRLTSVQHV--DRILVIEKGRIA

PG1048 NGENLSGGERQRISIARALLKDAPIVLLDEATASLDVENETLIQAGISELIKN--KTVLIIAHRMRTVANA--HKIVVLKEGTVA

PG1497 RGGKLSGGQRQRISIARAILKNPPILILDEATSALDTESERLVQNAIEKLMAN--RTAIVIAHRLTTIRNA--DIICVLHEGRIV

PG0903 --GKVSQGQKKKAVIALALSLRTPLLLMDEPTNGLDIPSKSAFRRLMARYITE-EQTVIISTHQVRDLEQII-DRIVMMDKNAIV

PG0844 KVEELSKGMQQKVQFVCTVIHEPELLIFDEPFSGFDPVNADLLKREILELAGK-ERTVIFSTHNMQSVEEVC-DDFALINKSRVV

PG1467 LIRDFSKGNQKKIGIAAAMLSRPDILILDEPFTALDPSSQIRLKKMLNEHATQYGTTMLISSHDLNHVTEVS-SRIVVLQKGEII

PG1923-N PTADFSGGWRMRIELAKLLLQRPEVLLLDEPTNHLDIESIGWLEQFIATNAGA----VILVSHDRAFIDNTT-TRTIEIELGHIY

PG1929-N LMKDLSGKQKVRVLLARALYGKPDNLLLDEPTNDLDLETVSWLENYLSNFENT----VLVVSHDRHFLDSVC-THTVDIDFGKVQ

PG1084-N -TDNLSGGELKRIALAGTLIGNPELLFLDEPTNHLDLDVIEWLEGYLSHQQIG----LLLVTHDRYFLDRVC-NRILELDNKQLY

PG1923-C KVSVLSGGERARLAIIRLLLQPANFLILDEPTNHLDMRSKDVLKEAIKNFDGT----VIVVSHDREFLDGLV-SKVYEFADGQVN

PG1929-C KASVLSGGEKMRCMISKMMMLDANTLVLDTPTNHLDLESIQAFNNTLKTYRGN----VLFASHDHEFIQTVA-NRIIELTPNGII

PG1084-C PIHKLSGGEQRRLYLCTVLMKNPNFLILDEPTNDLDILTLNVLENYLSEFKGC----VLVVSHDRFFMDKVV-NHLFTFEGNGIV

PG0233 VNEGFSGGEKKRNEIFQMAMLEPKLAILDETDSGLDIDALRIVAGGVNRLRSP-ENAAIVITHYQRLLEYIKPDFVHVLYKGRIV

Signature Walker B Downstream region